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KMID : 1144120110010020089
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Biomedical Engineering Letters
2011 Volume.1 No. 2 p.89 ~ p.98
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Neuronal micro-culture engineering by microchannel devices of cellular scale dimensions
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Goyal Gaurav
Nam Yoon-Key
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Abstract
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Purpose: The purpose of the current study was to investigate the effect of microchannel geometry on neuronal cultures and to maintain these cultures for long period of time (over several weeks) inside the closed microchannels of cellular scale dimensions.
Methods: The primary hippocampal neurons from E-18 rat were cultured inside the closed polydimethylsiloxane (PDMS) microchannels of varying sizes. The effect of the channel geometry on the spatial and the temporal variations in the neural microenvironment was investigated by studying neural maturation and variation in the media osmolality respectively. The cultures were maintained for longer time spans by PDMS device pretreatment, control on media evaporation (by using hydrophobic ethylene propylene membrane) and an effective culture maintenance protocol. Further, the devices were integrated with the planar microelectrode arrays (MEA) to record spontaneous electrical activity.
Results: A direct influence of channel geometry on neuron maturation was observed with cells in smaller channels maturing faster. The temporal variation in the microenvironment was caused by several fold increase in osmolality within 2?3 days due to rapid media evaporation. With our culture methodology, neurons were maintained in the closed channels as small as 50 μm in height and width for over 1 month in serum free media condition and the time varying spontaneous electrical activity was measured for up to 5 weeks using the MEA.
Conclusions: The understanding of the effect of the culture scale on cellular microenvironment and such long-term culture maintenance will be helpful in studying neuronal tissue development; therapeutic drug screening; and for network level neuronal analysis.
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KEYWORD
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Hippocampal neurons, Neuron maturation, PDMS Microfluidic channels, Long term culture, Electrophysiology
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